Figure 5. METTL3 and 14 overexpression leads to increased proliferation of transformed and immortal HMECs.

Transformed (A) and immortalized (B) HMECs were transfected with either a control GFP expressing plasmid or plasmids overexpressing METTL3 and METTL14 (M3/14). 48 hours after transfection, cells were re-plated at 0.5 × 10⁶ cells/well of a 6 well plate and allowed to recover for 16 hours. Cells were then detached by trypsin at each time point (0, 24, or 48 hours), and counted in a hemocytometer to determine cell number. ^*P ≤ 0.05 by paired Student's t-test. Error bars represent SEM of 3 experiments.