Procedure | Steps | Solution | Time/Temperature |
Dehydration | 1 | 70% alcohol | 15 min/RT |
2 | 80% alcohol | 15 min/RT | |
3 | 95% alcohol | 15 min/RT | |
4 | 100% alcohol | 15 min/RT | |
Clearing | 1 | Xylene | 60 min/4 °C |
2 | Xylene | 10 min/RT | |
3 | Xylene | 10 min/RT | |
4 | Xylene | 10 min/RT | |
*RT, room temperature |