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. 2018 May 20;(135):57369. doi: 10.3791/57369
Procedure Steps Solution Time/Temperature
Dehydration 1 70% alcohol 15 min/RT
2 80% alcohol 15 min/RT
3 95% alcohol 15 min/RT
4 100% alcohol 15 min/RT
Clearing 1 Xylene 60 min/4 °C
2 Xylene 10 min/RT
3 Xylene 10 min/RT
4 Xylene 10 min/RT
*RT, room temperature