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(A) The computational model predicts that I57L, Q12E, M81L, and A103V will have a neutral effect on the phosphotransfer, i.e., ΔHTCS ≈ 0. (B) The four mutants were observed to result in a decrease in phosphotransfer compared to the wild-type EnvZ/Spo0F interaction. Of these four mutations, only A103V was not found to significantly destabilize Spo0F (Table 1).
