Fig 7. Expression of mTOR phosphorylation in developing podocytes.

A. Immunohistochemistry demonstrated predominant expression of phosphorylated mTOR, commencing at the S-shaped body stage in podocytes of the newborn mouse kidney (arrow). In capillary and mature stages, mTOR phosphorylation occurred in podocytes, including in foot processes. B. Double immunofluorescence and confocal microscopy for phosphorylated mTOR and podocalyxin. Colocalization of phosphorylated mTOR with podocalyxin was seen in the capillary and mature stages. Arrows indicate positive staining of phosphorylated mTOR in immature and mature podocytes. Use of rabbit IgG instead of the primary antibody (phospho-mTOR) resulted in no apparent positive staining. An enlarged view is displayed within the rectangle.