Skip to main content
. 2018 Aug 8;14(8):e1007572. doi: 10.1371/journal.pgen.1007572

Fig 1. Cse4 preferentially targets to centromeres and functionally substitutes Cnp1 in fission yeast.

Fig 1

A, Cells carrying pREP1-CSE4-GFP that were induced for 24 hours show a single GFP focus (short). Multiple foci (commonly 3–6) were detected for 28-hour induction (long). (Bottom) Percentage of cells containing single or multiple focus. OE, overexpression. B, The single Cse4-GFP focus colocalizes with Sad1-CFP. C, Cse4-GFP partially rescues cnp1-1 growth defects at 36°C. Serial dilutions of indicated strains were plated in minimal medium without thiamine. Dilution = 10. D, Multiple GFP foci in cells overexpressing Cse4-GFP colocalize with mCherry-Swi6. E. ChIP qPCR showing relative enrichment of Cse4-GFP to centromeric region (cnt3), peri-centromeric region (otr) and sub-telomeric region (subT). ChIP was repeated in triplicate. Error bar indicates SEM. *, p<0.01. F, in situ chromatin-binding assay for wild type cells expressing Cse4-GFP. The heterochromatin mutant dos1Δ carrying GFP-Swi6 was used as a control (Bottom). GFP-Swi6 is dissociated from heterochromatin in dos1Δ, and was readily washed away by Triton X-100 as expected. Scale bars: 2 μm.