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. 2018 Aug 8;14(8):e1007572. doi: 10.1371/journal.pgen.1007572

Fig 6. Heterochromatin promotes centromeric targeting of Cnp1 by preventing ubiquitin-mediated degradation.

Fig 6

A, clr4Δ colonies expressing Cnp1-GFP under its native promoter obtained by crossing exhibited a diffuse GFP signal or single focus. (Right) Percentage of colonies displaying diffuse GFP or a single focus, and quantifications were based on random colony analysis. Scale bar: 2 μm. B, Western blot analysis of clr4Δ cells expressing Cnp1-GFP. WT was used as control. C, Stability assays for WT and clr4Δ cells expressing Cnp1-GFP. D, Extracts from indicated cells were analyzed by TUBE assays. C,D, Induction time:20 hours for WT and 22 hours for clr4Δ.