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. 2018 May 28;46(14):e86. doi: 10.1093/nar/gky410

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Production of a low-density multi-RNA array. (A) Generation of a Cy3-labelled MicA_sa, hapR_sa and MG_sa RNA array (i) Schematic layout of a DNA template array of MicA, MicA_sa, hapR, hapR_sa, MG and MG_sa. (ii) Cy3-labelled RNA array generated from the DNA template array following in vitro transcription.(B) Probing a low-density multi-RNA array with malachite green. (i) Schematic representation of the middle row of a non-labelled RNA array generated using the DNA template array in A(i). (ii) As in B(i) following probing with malachite green. (iii) A non-labelled MicA_sa, hapR_sa and MG_sa RNA array (i) (generated as in (A) but without labelling) probed with malachite green. The RNA array images have been mirror imaged so that the spot positions correspond to those shown in the schematics of the DNA template array.