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. 2018 May 28;46(14):e86. doi: 10.1093/nar/gky410

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Production of a high-density MicA_sa RNA array. (A) Preparation of a high-density DNA template array of MicA and MicA_sa. (i) Schematic layout of a single 6 × 6 grid with alternating rows of MicA and MicA_sa templates. (ii) A single 6 × 6 grid of a DNA template array of Alexa647-labelled MicA and MicA_sa. Individual spots were separated by 750 μm. (iii) The entire DNA template array of 21 repeating 6 × 6 grids as shown in (ii). (B) Production of a high-density MicA_sa RNA array from the DNA template array described in (A). (i) Schematic of the expected layout of a single grid of the MicA_sa RNA array. (ii) A single grid of the Cy3-labelled MicA_sa RNA array. (iii) The entire MicA_sa RNA array of 21 repeating grids as shown in (ii). The RNA array images have been mirror imaged so that the spot positions correspond to those shown in the schematics of the DNA template array.