Figure 2.

Amino acid sequences of the amyloplast stromal 112-kD protein and alignment with amino acid sequences of plastidic and cytosolic forms of potato SP. The isolated 112-kD protein was subjected to SDS-PAGE. The protein was then transferred to polyvinylidene difluoride paper and subjected to N-terminal amino acid sequence analysis. Another sample of the protein was digested with trypsin, three peptides were isolated, and subjected to amino acid sequence analysis. Sequences of the N terminus and from three internal peptides of the 112-kD protein were aligned with published sequences of plastidic SP (Nakano and Fukui, 1986; Nakano et al., 1989) and cytosolic SP (Mori et al., 1991) from potato tuber. The numbers in the figure represent the residue numbers that begin with the indicated published sequences.