Figure 3.

Dependence of the relative mobility of the 112-kD stromal SP on the concentration of amylopectin and glycogen upon affinity electrophoresis. Samples (30 μg) of the amyloplast stromal fraction were subjected to native PAGE in the presence of the indicated concentrations of amylopectin (A) and glycogen (B). Following electrophoresis, SP activity was measured by iodine staining. The relative mobility was calculated by dividing the migration of the activity band by the migration of the dye front. The inset of A and B contains a portion of the native gels showing the relative mobility of SP. The data shown in A and B is representative of two independent experiments.