iCM Media
Name of Reagent	Volume (mL)	Final Concentration
DMEM High Glucose	320
Medium 199	80
Fetal Bovine Serum	50	10%
Donor Horse Serum	25	5%
MEM Essential Amino Acids, 50x	10	1x
Sodium Pyruvate Solution, 100x	5	1x
MEM Non-Essential Amino Acids, 100x	5	1x
MEM Vitamin Solution, 100x	5	1x
Insulin-Transferrin-Selenium, 100x	5	1x
B27, 50x	10	1x
Penicilin-Streptomycin	5.5	1.1%
L-Glutamine supplement	5.5	1.1%
PE Media
Name of Reagent	Volume (mL)	Final Concentration
DMEM High Glucose	450
Fetal Bovine Serum	50	10%
Penicilin-Streptomycin	5.5	1.1%
L-Glutamine supplement	5.5	1.1%
Blasticidin-HCl - 10mg/mL	0.5	10 µg/mL
Puromycin dihydrochloride	0.05	1 µg/mL
Growth Media
Name of Reagent/ Equipment	Volume (mL)	Final Concentration
DMEM High Glucose	450
Fetal Bovine Serum	50	10%
Penicilin-Streptomycin	5.5	1.1%
L-Glutamine supplement	5.5	1.1%