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. 2018 Jun 18;46(14):7169–7178. doi: 10.1093/nar/gky525

Figure 3.

Figure 3.

Uracil excision by hUNG2 or its catalytic domain with junction DNA substrates containing one or two uracils. (A) Image of hUNG2 reaction products using the indicated U9–U33 junction substrates with or without a 32 nt 5′ overhang. The substrate concentration (0.1 μM) is 30-fold lower than in Figures 1 and 2, and the hUNG2 concentration was 180 pM. (B) Steady-state kinetic measurements for hUNG2 acting on the indicated junction substrates with a single U/A bp (U9 or U33). (C) Steady-state kinetic measurements for hUNG2 acting on duplex substrates without overhangs and containing a single U/A bp (U9 or U33). (D) Image of reaction products derived from the reaction of the catalytic domain with the indicated U9–U33 junction substrates with or without a 32 nt 5′ overhang. The substrate concentration was 0.1 μM, and the concentration of the catalytic domain was 540 pM. (E) and (F), Steady-state kinetic measurements for the catalytic domain acting on the indicated junction substrates with a single U/A bp (U9 or U33).