Figure 3.

Activity of the tfb3 promoter after UV irradiation using the lacS reporter gene assay. MW001-tfb3p was grown in a shaking flask in Brock media supplemented with 0.1% (w/v) NZ- amine, 0.2% (w/v) dextrin at 75°C until an OD₆₀₀ of 0.2–0.4 was reached. The control sample was taken before treatment. Afterwards, the remaining cells were split in 10 ml portions and the UV treatment was carried out in petri dishes. Subsequently, growth was continued in shaking flasks at 75°C. Specific β-galactosidase activities of samples before (control) and 45, 90 and 180 min after the treatment with (+UV, 100 J/m²) or without (−UV) UV irradiation were determined using ONPG as substrate and are given as specific activities (U mg⁻¹ protein). Medians were calculated from three biological replicates (n = 3) and error bars represent the respective standard deviation.