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ACE reporter activation through Cas9 nucleolytic cleavage. (A) Quantification of ACE reporter activation in 293T cells 72 h after co-transfection of ACE reporter, mCherry codon 59 targeting gRNA or NS-gRNA, and A3A-Cas9n-UGI, A3Bctd-Cas9n-UGI, rat APOBEC1-Cas9n-UGI/BE3, Cas9, or Cas9n expression constructs (n = 3; mean ± SD). (B) Anti-Cas9 and anti-HSP90 immunoblots from a representative experiment reported in panel A.
