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. 2018 Jul 6;46(14):6935–6949. doi: 10.1093/nar/gky594

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Structure of the destination vectors for use with the Candida albicans ORFeome. Schematic view of the 49 destination vectors, each designated with a standardized nomenclature, pCA-DESTijkl, whereby i stands for the transformation marker (1, URA3; 2, NAT1); j stands for the promoter (1, P_TET; 2, P_PCK1; 3, P_TDH3; 4, P_ACT1); k stands for N-terminal tagging (0, no tag; 1, 3xHA; 2, GFP; 3, TAP); and l stands for C-terminal tagging (0, no tag; 1, 3xHA; 2, GFP; 3, TAP). The spacer sequence (SP), represented by the yellow box, is intended to facilitate subsequent Illumina-based barcode sequencing. The Gateway cassette (GTW), represented by the green box, requires working with ccdB resistant Escherichia coli strains. Integration of StuI-linearized plasmids (or at the nearby I-SceI site if the cloned ORF contains StuI sites) is targeted at the RPS1 locus.