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. 2018 Jul 6;46(14):6935–6949. doi: 10.1093/nar/gky594

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Detection of 3xHA- or TAP-tagged Ume6 protein by western blot. Production of 3xHA-tagged (A) and TAP-tagged (B) Ume6 proteins. Candida albicans strains harboring the P_TET and P_TDH3 constructions were grown in YPD ± ATc3 for 2 and 4 h, respectively. Whole cell extracts were separated by SDS-PAGE and probed with a peroxidase-coupled antibody, allowing the detection of the 3xHA-tagged and TAP-tagged Ume6 protein. The tagged Ume6 proteins are indicated by an arrow along with their deduced sizes. M1: PageRuler Prestained Protein Ladder (Thermo Scientific) and M2: Precision Plus Protein™ Dual Color Standards (Bio-Rad).