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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Arthritis Rheumatol. 2018 Mar 25;70(5):746–755. doi: 10.1002/art.40414

Figure 5.

Figure 5.

Activation of autophagy by rapamycin reduces free HLA-B27 heavy chains on the cell surface. BM macrophages were incubated without or with IFNγ (50 ng/ml) for 24 hours, and treated without or with rapamycin (25 μM) for the last 2 (Ra-2h) or 4 hours (Ra-4h). (A) Cells were then stained with APC-conjugated HC10 to detect free heavy chains by flow cytometry. (B) For the detection of folded HLA-B27, cells were stained with Pacific Blue-conjugated ME1. Quantitative results are mean ± SEM from four independent experiments (**, p < 0.005).