Table 2.
Primers used for Real-Time quantitative PCR. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MAPK1, Mitogen-activated protein kinase 1 (ERK2); MAPK9, Mitogen-activated protein kinase 9 (JNK2); MAPK14, Mitogen-activated protein kinase 14 (p38); c-Jun, AP-1 transcription factor OC, osteocalcin; BSP, Bone Sialoprotein; MMP2, Matrix metalloproteinase-2.
| Primer | Sequence (5′-3′) | Tm (°C) |
|---|---|---|
| GAPDH | F- ACCCAGAAGACTGTGGATGG | 60 |
| R- CAGTGAGCTTCCCGTTCAG | ||
| Integrin αV | F- AATCTTCCAATTGAGGATATCAC | 60 |
| R- AAAACAGCCAGTAGCAACAAT | ||
| Integrin β3 | F- CCGTGACGAGATTGAGTCA | 60 |
| R- AGGATGGACTTTCCACTAGAA | ||
| MAPK1 | F- CTGGGTATTCTTGGATCTCC | 60 |
| R- GCCTGTTCAACTTCAATCCT | ||
| MAPK9 | F- ATGGGAGCAGGTGAGACG | 60 |
| R- TGTTTCAGACACGGACTTGG | ||
| MAPK14 | F- GGTTACGTGTGGCAGTGAAG | 60 |
| R- AACGTCCAACAGACCAATCA | ||
| c-Jun | F- GCATGAGGAAACGCATCGCTGCCTCCAAGT | 60 |
| R- GCGACCAAGTCCTTCCCACTCGTGCACACT | ||
| Runx-2 | F- ATGCTTCATTCGCCTCAC | 60 |
| R- ACTGCTTGCAGCCTTAAAT | ||
| OC | F- ATGAGAGCCCTCACACTCCTCG | 60 |
| R- GTCAGCCAACTCGTCACAGTCC | ||
| BSP | F- ATGGCCTGTGCTTTCTCAATG | 60 |
| R- GGATAAAAGTAGGCATGCTTG | ||
| MMP2 | F- AGATCTTCTTCTTCAAGGACCGGTT | 60 |
| R- GGCTGGTCAGTGGCTTGGGGTA |