| Component | Stock | Final volume for 20 μL reaction |
| Template (RNA) | 50 ng/µL | 1.0 μL |
| Reaction buffer | 5x | 4.0 μL |
| Oligo dT primer | Master stock | 2 μL |
| Reverse Transcriptase | Master stock | 1 μL |
| Nuclease-free distilled water | - | 12 μL |
| Total Reaction Volume | 20 μL |
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