| Component | Stock | Final volume for 10 μL reaction |
| cDNA product | - | 1.0 μL |
| iTaq Universal SYBR mix | 2x | 5.0 μL |
| Target (ORF/3’ UTR) F.P. | 10 μM | 0.5 μL |
| Target (ORF/3’ UTR) R.P. | 10 μM | 0.5 μL |
| Nuclease-free distilled water | - | 3 μL |
| Total Reaction Volume | 10 μL |
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