| 95 °C = 10 min |
| 30 cycles of: |
| 95 °C = 10 s |
| 56 °C = 30 s |
| 72 °C = 30 s |
| Melt Curve Analysis |
| 65 °C= 31 s |
| Linear Ramp rate = 0.5 °C/s |
| Acquisition = 0.5 °C intervals |
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