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. 2018 Jun 13;(136):57664. doi: 10.3791/57664
Sendai Virus Primers
Target Primer Sets
SeV Forward: GGATCACTAGGTGATATCGAGC
Reverse: ACCAGACAAGAGTTTAAGAGATATGTATC
KOS (KLF4/OCT4/SOX2) Forward: ATGCACCGCTACGACGTGAGCGC
Reverse: ACCTTGACAATCCTGATGTGG
KLF4 Forward: TTCCTGCATGCCAGAGGAGCCC
Reverse: AATGTATCGAAGGTGCTCAA
c-MYC Forward: TAACTGACTAGCAGGCTTGTCG
Reverse: TCCACATACAGTCCTGGATGATGATG
RT-PCR Primers
Target Primer Sets
NANOG Forward: TTTGTGGGCCTGAAGAAAACT
Reverse: AGGGCTGTCCTGAATAAGCAG
SOX2 Forward: AGAAGAGGAGAGAGAAAGAAAGGGAGAGA
Reverse: GAGAGAGGCAAACTGGAATCAGGATCAAA
OCT4 Forward: AACCTGGAGTTTGTGCCAGGGTTT
Reverse: TGAACTTCACCTTCCCTCCAACCA
DPPA4 Forward: GACCTCCACAGAGAAGTCGAG
Reverse: TGCCTTTTTCTTAGGGCAGAG
REX1 Forward: GCCTTATGTGATGGCTATGTGT
Reverse: ACCCCTTATGACGCATTCTATGT
ALPL Forward: GGGACTGGTACTCAGACAACG
Reverse: GTAGGCGATGTCCTTACAGCC
COL1A1 Forward: GTGCGATGACGTGATCTGTGA
Reverse: CGGTGGTTTCTTGGTCGGT
PTH1R Forward: AGTGCGAAAAACGGCTCAAG
Reverse: GATGCCTTATCTTTCCTGGGC
BGLAP Forward: GGCGCTACCTGTATCAATGG
Reverse: GGCGCTACCTGTATCAATGG