Fibroblast Medium (500 mL)
DMEM	440 mL
Heat-inactivated FBS	50 mL
Antibiotics(Pen/Strep) (100x)	5 mL
Nonessential Amino Acid (100x)	5 mL
2-Mercaptoethanol	3.5 µL
MEF/MSC Culture Medium (500 mL)
DMEM	440 mL
Heat-inactivated FBS	50 mL
Antibiotics(Pen/Strep) (100x)	5 mL
L-Glutamine (100x)	5 mL
hESC medium (500 mL)
DMEM/F-12	384.5 mL
KnockOut Serum Replacement	100 mL (total: 20%)
Nonessential Amino Acid (100x)	5 mL
Antibiotics (Pen/Strep) (100x)	5 mL
L-Glutamine (100x)	5 mL
bFGF (10 µg/mL)	500 µL
2-Mercaptoethanol	3.5 µL
MSC Differentiation Medium (500 mL)
KnockOut DMEM/F-12 or DMEM/F-12	445 mL
KnockOut Serum Replacement	50 mL
bFGF (10 µg/mL)	500 µL (10 ng/mL)
PDGF-AB (25 µg/mL)	200 µL (10 ng/mL)
Nonessential Amino Acid (100x)	5 mL
2-Mercaptoethanol	3.5 µL
Osteoblast Differentiation Medium (ODM) (500 mL)
αMEM	395 mL
Heat-inactivated FBS	50 mL
10 mM β-Glycerophosphate	50 mL (1.08 g in 50 mL αMEM)
0.1 µM Dexamethasone (Light Sensitive)	10 µL of 5 mM dexamethasone
200 µM Ascorbic Acid (Light Sensitive)	100 µL of 1 mM ascorbic acid
Antibiotics (Pen/Strep) (100x)	5 mL
Matrigel coating solution (50 mL)
Basement Membrane Matrix	2 mL
DMEM/F-12 (pre-cold 4 °C)	48 mL
Osteoblast Detachment Solution (50 mL)
0.25% Trypsin-EDTA	25 mL
Collagenase II Solution (1 mg/mL)	25 mL
Note: Prepare osteoblast detachment solution fresh right before use. Collagenase II solution is stored at -20 °C for up to 6 months.