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. 2018 Jun 19;(136):57688. doi: 10.3791/57688
384 well 96 well 12 well 6 well 100 mm 150 mm
Coating volume (per well) 10 µL 50 µL 500 µL 1 mL 5 mL 10 mL
Medium volume (per well) 100 µL 300 µL 2 mL 5 mL 12 mL 30 mL
Differentiation day 0 activin A concentration (ng/mL) 100 100 100 300
Cardiac progenitor day 5 yield (cells/well) 1.0 – 1.2 x 105 2.5 – 3.0 x 105 1.0-1.5 x 107 2.0 – 3.0 x 107
Cardiac progenitor day 5 replating or thawing density (cells/well) 2.0 x 104 7.5 x 105 4.5 x 106 8.0  x 106 2.2 x 107
Ventricular-like cardiomyocyte day 14–16 yield (cells/well) 2.0 – 5.0 x 104 0.8 – 1.2 x 106 3.5 – 5.0 x 106 0.8 – 1.2 x 107 1.8 – 2.5 x 107
Ventricular-like cardiomyocyte replating density (cells/well) 2.5 x 104 1.0 x 106 4.5 x 106 1.0 x 107 2.2 x 107