384 well | 96 well | 12 well | 6 well | 100 mm | 150 mm | |
Coating volume (per well) | 10 µL | 50 µL | 500 µL | 1 mL | 5 mL | 10 mL |
Medium volume (per well) | 100 µL | 300 µL | 2 mL | 5 mL | 12 mL | 30 mL |
Differentiation day 0 activin A concentration (ng/mL) | 100 | 100 | 100 | 300 | ||
Cardiac progenitor day 5 yield (cells/well) | 1.0 – 1.2 x 105 | 2.5 – 3.0 x 105 | 1.0-1.5 x 107 | 2.0 – 3.0 x 107 | ||
Cardiac progenitor day 5 replating or thawing density (cells/well) | 2.0 x 104 | 7.5 x 105 | 4.5 x 106 | 8.0 x 106 | 2.2 x 107 | |
Ventricular-like cardiomyocyte day 14–16 yield (cells/well) | 2.0 – 5.0 x 104 | 0.8 – 1.2 x 106 | 3.5 – 5.0 x 106 | 0.8 – 1.2 x 107 | 1.8 – 2.5 x 107 | |
Ventricular-like cardiomyocyte replating density (cells/well) | 2.5 x 104 | 1.0 x 106 | 4.5 x 106 | 1.0 x 107 | 2.2 x 107 |