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. 2018 Aug 13;7:e36588. doi: 10.7554/eLife.36588

Figure 2. RNST-2 is widely expressed and localizes to lysosomes.

Figure 2.

(A–H′) DIC and confocal fluorescence images of wild type expressing RNST-2::CHERRY driven by the rnst-2 promoter. RNST-2::CHERRY is expressed from early embryos (A, A’) to the adult stage in various cell types including hypodermis (B, B’), intestine (C, C’), sheath cell (D, D’), pharynx (E, E’), tail (F, F’), vulva (G, G’) and muscle cell (H, H’). (I–J″) Confocal fluorescence images of the hypodermis in wild type co-expressing RNST-2::CHERRY and LAAT-1::GFP (I–I″) or SCAV-3::GFP (J–J″). RNST-2 colocalizes with LAAT-1 and SCAV-3 to both globular (arrowheads) and tubular (arrows) lysosomes. Scale bars: 5 µm.