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. 2018 Aug 13;7:e36588. doi: 10.7554/eLife.36588

Figure 6. RNST-2 maintains pyrimidine availability for embryonic development.

The percentage of viable embryos was scored in the indicated strains without (A, B) or with uridine (C, D) or cytidine supplementation (E, F). At least 150 embryos were examined in each strain and three independent experiments were performed. Data are shown as mean ± SD. One-way ANOVA with Tukey’s post hoc test was performed to compare all other datasets with wild type or datasets that are linked by lines. *p<0.05, **p<0.001, ***p<0.0001, N.S.: no significance.

Figure 6—source data 1. Figure 6 numerical data.
elife-36588-fig6-data1.xlsx (18.3KB, xlsx)
DOI: 10.7554/eLife.36588.023
Figure 6—source data 2. Figure 6—figure supplement 1 numerical data.
elife-36588-fig6-data2.xlsx (23.2KB, xlsx)
DOI: 10.7554/eLife.36588.024
Figure 6—source data 3. Figure 6—figure supplement 2 numerical data.
elife-36588-fig6-data3.xlsx (17.4KB, xlsx)
DOI: 10.7554/eLife.36588.025

Figure 6.

Figure 6—figure supplement 1. Loss of RNST-2 function but not a general lysosomal defect leads to synthetic embryonic lethality in pyr-1 and umps-1 mutants.

Figure 6—figure supplement 1.

(A–E) The percentage of viable embryos (A–D) and L1 larvae growing to L4 (E) was quantified in the indicated strains. At least 150 embryos or 100 larvae were examined in each strain and at least three independent experiments were performed. (F–I) Lifespan analyses were performed in the indicated strains. More than 100 worms were examined in each strain and three independent experiments were performed. The mean lifespan in the indicated strains was quantified and is shown in (G, I). In (A–E, G, I), data are shown as mean ± SD. One-way ANOVA with Tukey’s post hoc test (A–D, G, I) or Student’s two-tailed unpaired t test (E) was performed to compare datasets with wild type or datasets that are linked by lines. *p<0.05, **p<0.001, ***p<0.0001, N.S.: no significance.
Figure 6—figure supplement 2. Autophagy is important for maintaining pyrimidine availability during embryonic development.

Figure 6—figure supplement 2.

The percentage of viable embryos was quantified in the indicated strains without (A, B) or with uridine (C) or cytidine (D) treatment. At least 150 embryos were examined in each strain/treatment and at least three independent experiments were performed. One-way ANOVA with Tukey’s post hoc test (A, B) or Two-way ANOVA with the Bonferroni post hoc test (C, D) was performed to compare datasets with wild type or datasets that are linked by lines. *p<0.05, **p<0.001, ***p<0.0001, all other points had p>0.05.