Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (C. elegans) |
rnst-2 | WBGene00019624 | Other name: CELE_K10C9.3 |
|
| rpl-5 | WBGene00004416 | Other name: CELE_F54C9.5 |
||
| rpl-25.2 | WBGene00004439 | Other name: CELE_F52B5.6 |
||
| rps-3 | WBGene00004472 | Other name: CELE_C23G10.3 |
||
| rps-0 | WBGene00004469 | Other name: CELE_B0393.1 |
||
| Gene (human) | rnaset2 | National Center for Biotechnology Information |
Gene ID: 8635 | |
| Strain (C. elegans) |
N2 | CGC | RRID:WB-STRAIN:N2_(ancestral) | wild type (Bristol) |
| XW1624 | DOI: 10.1126/science.1220281 | laat-1(qx42) | ||
| XW5331 | DOI: 10.1016/j.cell.2010.04.034 | lgg-1(bp500) | ||
| OK286 | DOI: 10.1042/BJ20131085 | RRID:WB-STRAIN:OK286 | pyr-1(cu8) | |
| HZ1690 | DOI 10.1016/j.devcel.2011.06.024 | RRID:WB-STRAIN:HZ1690 | epg-6(bp242) | |
| SP507 | DOI: 10.1042/BJ20131085 | RRID:WB-STRAIN:SP507 | umps-1(mn160) | |
| CF1041 | DOI: 10.1126/science.1139952 | daf-2(e1370) | ||
| HZ1687 | DOI: 10.1083/jcb.201209098 | RRID:WB-STRAIN:HZ1687 | atg-9(bp564) | |
| VC893 | DOI: 10.1016/j.cell.2010.04.034 | RRID:WB-STRAIN:VC893 | atg-18(gk378) | |
| XW10781 | this paper | rnst-2(qx245) | ||
| XW15104 | this paper | rnst-2(bp555) | ||
| HZ1683 | DOI 10.1016/j.devcel.2011.06.024 | RRID:WB-STRAIN:HZ1683 | atg-2(bp576) | |
| CF1903 | CGC | RRID:WB-STRAIN:CF1903 | glp-1(e2144) | |
| XW5097 | RRID:WB-STRAIN:PD4482 | lmp-1(nr2045) | ||
| XW7251 | DOI: 10.1126/science.1220281 |
qxIs354
(Pced-1LAAT-1::GFP) |
||
| XW5399 | DOI: 10.1126/science.1220281 |
qxIs257
(Pced-1NUC-1::CHERRY) |
||
| XW8056 | DOI: 10.1083/jcb.201602090 |
qxIs430
(Pscav-3SCAV-3::GFP) |
||
| XW10197 | DOI: 10.1083/jcb.201602090 |
qxIs468
(Pmyo-3LAAT-1::GFP) |
||
| XW642 | DOI: 10.1091/mbc.10.12.4311 |
bIs1
(Pvit-2VIT-2::GFP) |
||
| RT688 | DOI: 10.1126/science.1220281 |
pwIs281
(Ppie-1CAV-1::GFP) |
||
| XW8293 | DOI: 10.15252/embr.201438618 |
qxEx4098
(Pced-1GFP:ATG-18) |
||
| XW12615 | this paper |
qxIs544
(Prnst-2RNST-2::CHERRY) |
||
| XW13567 | this paper |
qxEx6614
(Prnst-2RNST-2(H118A)::CHERRY) |
||
| XW8783 | this paper |
qxEx4279
(Pmyo-3LAAT-1::GFP) |
||
| XW17778 | this paper |
qxEx8302
(Prnst-2RNASET2-CHERRY) |
||
| Antibody | anti-RPL-5 (rat polyclonal) |
this paper | WB(1:300) | |
| anti-RPL-25.2 (rat polyclonal) |
this paper | WB(1:300) | ||
| anti-RPS-0 (rat polyclonal) |
this paper | WB(1:1000) | ||
| anti-RPS-3 (rat polyclonal) |
this paper | WB(1:1000) | ||
| anti-CPL-1 (rat polyclonal) |
DOI: 10.1126/science.1220281 | WB(1:500) | ||
| anti-LGG-1 (rat polyclonal) |
DOI: 10.1016/j.cell.2010.04.034 | WB(1:1000); Immunostaining(1:1000) |
||
| anti-alpha-Tubulin (mouse monoclonal) |
Sigma-Aldrich (Missouri, USA) | Cat #T5168; RRID:AB_477579 |
WB(1:10000) | |
| anti-SQST-1 (rat polyclonal) |
DOI: 10.1016/j.cell.2010.04.034 | Immunostaining (1:1000) |
||
| anti-PGL-3 (rat polyclonal) |
DOI: 10.1016/j.cell.2010.04.034 | Immunostaining (1:1000) |
||
| anti-SEPA-1 (rabbit polyclonal) |
DOI: 10.1016/j.cell.2010.04.034 | Immunostaining (1:1000) |
||
| anti-HSP-60 (mouse polyclonal) |
DOI: 10.1126/science.1220281 | WB(1:1000) | ||
| anti-HEL-1 (rabbit polyclonal) |
DOI: 10.1126/science.1220281 | WB(1:500) | ||
| anti-RME-1 (mouse polyclonal) |
DOI: 10.1126/science.1220281 | WB(1:1000) | ||
| Plasmids for generating transgenic strains |
pPD49.26-Prnst-2RNST- 2::CHERRY |
this paper | Cloning described in 'Plasmid construction' |
|
| pPD49.26-Prnst-2
RNASET2::CHERRY |
this paper | Cloning described in 'Plasmid construction' |
||
| pPD49.26-Prnst-2
RNST-2(H118A)::CHERRY |
this paper | Cloning described in 'Plasmid construction' |
||
| pPD49.26-Prnst-2RNST- 2(cDNA)::CHERRY |
this paper | Cloning described in 'Plasmid construction' |
||
| Plasmids for protein expression |
pET-21b-rpl-25.2 | this paper | Cloning described in 'Plasmid construction' |
|
| pET-21b-rpl-5 | this paper | Cloning described in 'Plasmid construction' |
||
| pET-21b-rps-0 | this paper | Cloning described in 'Plasmid construction' |
||
| pET-21b-rps-3 | this paper | Cloning described in 'Plasmid construction' |
||
| Commercial assay or kit | Lysosome Isolation Kit | Sigma-Aldrich (Missouri, USA) | Cat #LYSISO1 | |
| RNeasy Plus Universal Mini Kit |
QIAGEN (Hilden, Germany) | Cat #73404 | ||
| Chemical compound, drug | Uridine | Sigma-Aldrich (Missouri, USA) | Cat #U3003 | |
| Cytidine | Sigma-Aldrich (Missouri, USA) |
Cat #C4654 | ||
| DAPI | Vector Laboratories (California, USA) |
Cat #H-1200; RRID:AB_2336790 |
||
| LysoTracker red | Invitrogen (Oregon, USA) |
Cat #L7528 | ||
| Software, algorithm | Velocity | PerkinElmer (Massachusetts, USA) |
||
| Zen | Carl Zeiss (Oberkochen, Germany) |
RRID:SCR_01367 |