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. 2018 Jun 20;(136):57860. doi: 10.3791/57860
Plasmids Relevant characteristics Reference
pAH7 Pnat parB-yfp;Mx8 attP; TetR 19
pAH53 PpomZ mCherry-pomX; Mx8 attP ; KmR 16
pDS150 1 Pnat ftsZ-gfp ; mxan18-19 ; TetR This study
pMR3691 Plasmid for vanillate inducible gene expression 18
pKA51 Pnat ftsZ-gfp ; Mx8 attP; TetR 17
1 pDS150: pDS150 is a derivative of pKA51 in which the Mx8 attP site was replaced with the mxan18-19 intergenic region. For this the mxan18-19 intergenic region was amplified from pMR3691 with primers Mxan18-19 fwd BsdRI (GCGATCATTGCGCGCCAGACGATAACAGGC) and Mxan18-19 rev BlpI (GCGGCTGAGCCCGCGCCGACAACCGCAACC) and cloned into pKA51.