| Cycle Step | Temp. | Time | Cycles |
| Initial denaturation | 95 °C | 3 min | 1 |
| Denaturing | 95 °C | 30 sec | 36 |
| Annealing* | X °C | 30 sec | |
| Extension | 72 °C | 20 sec/kb | |
| Final Extension | 72 °C | 5 min | 1 |
| 4 °C | hold |
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