| Components | 20 μl reaction | 100 reactions | Final Conc. |
| Nuclease-free H2O | 7.45 μL | 745 μL | |
| Go-Taq 2x (Promega, M7122) | 10 μL | 1000 μL | 1x |
| primer Gen1_FW | 0.25 μL | 25 μL | 0.125 μM |
| primer 5 nt-ins-specific_FW | 0.3 μL | 30 μL | 0.15 μM |
| Primer Gen2_RV | 0.25 μL | 25 μL | 0.125 μM |
| Primer WT-specific_RV | 0.25 μL | 25 μL | 0.125 μM |
| DNA | 1.5 μL | -- |
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