| Operation | Temp. | Time | Cycles |
| Initial Denaturation | 95 °C | 3 - 5 min | 1 |
| Denaturation | 95 °C | 30 sec | 35 |
| Annealing | 55 °C | 30 sec | |
| Elongation | 72 °C | 40 sec | |
| Final elongation | 72 °C | 10 min | 1 |
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