Table 1.
Group-specific synovitis assessments, RNA and sequencing qualities, extent of synovial transcriptomes and quantitative cartilage assessment (surgical treatment groups pooled; see Supplementary Table 1 for the comparison of the surgical treatment groups; see Sieker et al. JOR 2017 for baseline characteristics including age, weight and sex).
| Scale | Intact (n=6) | 1W (n=18) | 4W (n=18) | 1W/Intact | 4W/Intact | 4W/1W | |
|---|---|---|---|---|---|---|---|
| Synovitis Assessments | Median (Range) | Median (Range) | Median (Range) | P value | P value | P value | |
| Macroscopic score (OARSI) | 0-5 | 1 (0, 2) | 2 (1, 3) | 2 (1, 3) | .125 | .140 | .753 |
| Microscopic sum (Krenn) | 0-9 | 0 (0, 1) | 4 (1, 8) | 3 (1, 8) | <.001 | .003 | .179 |
| Lining score | 0-3 | 0 (0, 1) | 2 (1, 3) | 1.5 (0, 3) | <.001 | .006 | .123 |
| Stromal cellularity score | 0-3 | 0 (0, 0) | 1 (0, 3) | 1 (0, 3) | .001 | .005 | .363 |
| Infiltration score | 0-3 | 0 (0, 0) | 0.5 (0, 2) | 0 (0, 2) | .097 | .307 | .313 |
| Mean (95% CI) | Mean (95% CI) | Mean (95% CI) | P value | P value | P value | ||
| Cellcount (in 1000 cells) | 0-∞ | 0.89 (-0.22, 2.00) | 8.80 (5.45, 12.16) | 7.15 (5.19, 9.11) | .002 | .002 | .860 |
| Tissue area (in million px) | 0-∞ | 0.36 (0.01, 0.71) | 3.77 (1.84, 5.70) | 2.43 (1.82, 3.03) | .002 | .002 | .892 |
| RNA and Sequencing quality | Mean (95% CI) | Mean (95% CI) | Mean (95% CI) | P value | P value | P value | |
| Concentration in ng/μl | |||||||
| Pooled | 0-∞ | 126.3 (66.8, 185.9) | 899.9 (712.3, 1087.6) | 1081.9 (867.7, 1296.2) | .005 | <.001 | .202 |
| ACLT only | 0-∞ | 903 (710, 1097) | 786 (560, 1010) | 0.239 | 0.431 | 1 | |
| RECON only | 0-∞ | 870 (538, 1203) | 1270 (764, 1776) | 0.239 | 0.0038 | 1 | |
| REPAIR only | 0-∞ | 926 (468, 1384) | 1191 (951, 1431) | 0.239 | 0.004 | 1 | |
| 260/280 absorbance ratio | |||||||
| Pooled | 0-∞ | 1.98 (1.81, 2.14) | 2.08 (2.06, 2.10) | 2.08 (2.06, 2.09) | .594 | .694 | .594 |
| ACLT only | 0-∞ | 2.1 (2.1, 2.1) | 2.1 (2.0, 2.1) | 1 | 1 | 1 | |
| RECON only | 0-∞ | 2.1 (2.0, 2.1) | 2.1 (2.0, 2.1) | 1 | 1 | 1 | |
| REPAIR only | 0-∞ | 2.1 (2.0, 2.1) | 2.1 (2.1, 2.1) | 1 | 1 | 1 | |
| 260/230 absorbance ratio | |||||||
| Pooled | 0-∞ | 1.67 (1.12, 2.23) | 2.35 (2.16, 2.53) | 2.20 (2.11, 2.30) | .081 | .209 | .407 |
| ACLT only | 0-∞ | 2.3 (2.3, 2.3) | 2.2 (2.1, 2.4) | 0.61 | 0.91 | 1 | |
| RECON only | 0-∞ | 2.2 (2.0, 2.4) | 2.3 (2.3, 2.3) | 1 | 1 | 1 | |
| REPAIR only | 0-∞ | 2.6 (2.0, 3.1) | 2.3 (2.3, 2.3) | 0.44 | 0.86 | 1 | |
| RNA integrity number (RIN) | |||||||
| Pooled | 7.5 (7.0, 7.9) | 8.4 (8.1, 8.8) | 8.2 (7.7, 8.6) | .027 | .065 | .509 | |
| ACLT only | 8.3 (7.6, 8.9) | 8.0 (7.2, 8.8) | 1 | 1 | 1 | ||
| RECON only | 8.4 (7.6, 9.2) | 8.2 (7.3, 9.1) | 0.955 | 1 | 1 | ||
| REPAIR only | 8.7 (8.2, 9.1) | 8.3 (7.8, 8.7) | 0.131 | 1 | 1 | ||
| Number of reads in millions | |||||||
| Pooled | 0-∞ | 17.4 (13.5, 21.3) | 17.5 (12.8, 22.2) | 14.4 (13.3, 15.4) | .635 | .635 | .635 |
| ACLT only | 0-∞ | 14.7 (12.0, 17.5) | 14.6 (12.8, 16.3) | 1 | 1 | 1 | |
| RECON only | 0-∞ | 15.9 (8.9, 22.8) | 15.3 (13.3, 17.3) | 1 | 1 | 1 | |
| REPAIR only | 0-∞ | 21.9 (9.8, 33.9) | 13.2 (11.6, 14.8) | 1 | 1 | 0.761 | |
| Reads uniquely mapped in % | |||||||
| Pooled | 0-100 | 83.1 (82.2, 84.1) | 79.5 (78.1, 80.9) | 81.2 (79.9, 82.5) | .028 | .206 | .206 |
| ACLT only | 0-100 | 78.3 (77.0, 79.6) | 78.7 (76.8, 80.6) | 0.052 | 0.95 | 1 | |
| RECON only | 0-100 | 77.1 (75.4, 78.7) | 80.6 (79.9, 81.4) | 0.011 | 0.99 | 0.853 | |
| REPAIR only | 0-100 | 83.1 (82.6, 83.6) | 84.4 (83.6, 85.1) | 1 | 1 | 1 | |
| Synovial Transcriptome | Mean (95% CI) | Mean (95% CI) | Mean (95% CI) | P value | P value | P value | |
| Transcripts >1RPKM in thousands | |||||||
| Pooled | 0-∞ | 12.6 (12.5, 12.8) | 12.8 (12.7, 12.9) | 12. 6 (12.4, 12.7) | .460 | .694 | .072 |
| ACLT only | 0-∞ | 12.8 (12.6, 13.0) | 12.4 (12.3, 12.5) | 1 | 1 | 0.372 | |
| RECON only | 0-∞ | 12.7 (12.4, 13.0) | 12.7 (12.5, 13.0) | 1 | 1 | 1 | |
| REPAIR only | 0-∞ | 12.9 (12.7, 13.1) | 12.5 (12.2, 12.8) | 1 | 1 | 1 | |
| Quantitative Cartilage Assessment | Mean (95% CI) | Mean (95% CI) | Mean (95% CI) | P value | P value | P value | |
| Safranin-O saturation | |||||||
| Pooled | 0-255 | 220 (217, 223) | 195 (182, 208) | 190 (175, 205) | 0.072 | 0.040 | 0.587 |
| ACLT only | 0-255 | 209 (191, 226) | 211 (196, 226) | 1 | 1 | 1 | |
| RECON only | 0-255 | 196 (178, 213) | 186 (177, 196) | 0.859 | 0.115 | 1 | |
| REPAIR only | 0-255 | 181 (156, 210) | 172 (134, 210) | 0.311 | 0.180 | 1 | |