Figure 2.
Hematopoiesis and neutrophil localization at homeostasis in WT and Rgs5−/− mice. A–D, serum hemoglobin (Hgb; A), platelet (B), total leukocyte (WBC; C), and differential leukocyte (D) counts were determined from peripheral blood. Error bars indicate mean ± S.E. A–C, *, p = 0.01, t test; n.s., not significant. D, **, p = 0.001; ***, p = 0.0008, two-way ANOVA, Sidak multiple comparisons. E–G, neutrophil numbers were determined in BM (tibia and femur of both lower extremities) (E), spleen (F), or lungs (G) by flow cytometry–based assessment of percentages of Ly6GhiCD11b+ cells, respectively, as shown in the representative plot from bone marrow. All results are mean ± S.E.; each symbol represents an individual mouse.