Figure 4.

Neutrophils from RGS5-deficient mice are intrinsically capable of enhanced migration. A, mice were injected i.p. with 4% sodium thioglycollate. At the indicated time points, total cells (left) and neutrophils (right) were determined by flow cytometry as in Figs. 1 and 2. Error bars indicate mean ± S.E. of six to eight mice/genotype evaluated in three to four experiments. *, p = 0.02; **, p = 0.004, two-way ANOVA, Sidak multiple comparisons; n.s., not significant. B, chemokine CXCL1 levels were evaluated in peritoneal fluid following TG injection. Results are mean ± S.E.; each symbol represents one mouse. C, surface CXCR2 in TG-elicited peritoneal neutrophils determined by flow cytometry using the antibodies as indicated (IgG2a, isotype control). Histograms are from a single mouse representative of four mice/group assessed in two to three independent experiments. D, BM-derived neutrophils were stained with violet (Rgs5^−/−) or green (WT) fluorophores. Cells were mixed at a 1:1 ratio and injected i.v. into mice previously administered TG. Numbers of cells of each genotype were determined by flow cytometry. Error bars indicate mean ± S.E. of cells from four mice/group evaluated in two independent experiments. ***, p = 0.0005, two-way ANOVA, Sidak multiple comparisons.