Figure 6.

RGS5 negatively regulates chemokine-induced Ca²⁺ signaling and Akt phosphorylation in neutrophils. A, Akt phosphorylation (pAkt) in BM-derived neutrophils was evaluated by immunoblotting. The blot is from a single experiment representative of seven similar experiments. Error bars indicate mean ± S.E. *, p = 0.03, Mann–Whitney. B–D, Ca²⁺ flux in response to CXCL1 (B), CXCL2 (C), or ionomycin (D). Bar graphs represent area under the curve (error bars are mean ± S.E.) of five to eight independent measurements in four separate experiments each using cells from one mouse of each genotype. *, p = 0.04, Mann–Whitney. The arrow represents time of agonist addition. n.s., not significant.