Figure 7.

RGS5-deficient neutrophils demonstrate increased chemokine-induced directional velocity and adhesion to endothelial cells. A and B, migrational velocity and directional error of BM-derived neutrophils were assessed in Dunn chambers in the presence of CXCL2 (10 μm; A) or fMLP (100 nm or 5 μm; B). Error bars indicate mean ± S.E. of three independent experiments using cells from one mouse/genotype and three to five separate chambers/condition in which 50–110 cells/chamber were individually tracked. ****, p < 0.00001, unpaired t test; **, p = 0.009, Mann–Whitney. C, neutrophil adhesion was assessed by binding to immobilized ICAM-1 as described under “Experimental procedures” in the presence of CXCL2 (10 μm) or fMLP (5 μm) in three independent experiments using cells from one mouse of each genotype. ***, p < 0.0005, one-way ANOVA, Sidak multiple comparisons. Error bars indicate mean ± S.E. D, EC adhesion was assessed in flow chambers as described under “Experimental procedures.” Error bars indicate mean ± S.E. of four independent experiments using cells from one mouse of each genotype. ****, p < 0.00001, unpaired t test. IL-8, interleukin-8.