Figure 8.

RGS5 overexpression inhibits chemotaxis of human neutrophils. A, human peripheral blood neutrophils were isolated and transduced with tat fusion proteins as described under “Experimental procedures.” Cell lysates were prepared, and intracellular expression of tat GFP fusion proteins was assessed by immunoblotting. B, chemotaxis of human neutrophils transduced with tat fusion proteins was assessed in Transwell assays. Error bars indicate mean ± S.E. of three independent experiments using neutrophils from one to two donors/experiment. **, p < 0.008, two-way ANOVA, Sidak multiple comparisons.