Clustering of matrix-detached cells influences the relative levels of ferroptosis and apoptosis in the absence of α6β4.
A, control and β4-depleted cells were detached for 24 h in the presence of either DMSO, ferrostatin-1 (2 μm), Z-VAD-fmk (25 μm), or ferrostatin-1 and Z-VAD-fmk, and the number of viable cells was quantified. B, control and β4-depleted cells were detached for 24 h with 2 mm EDTA in the presence of either DMSO, ferrostatin-1 (2 μm), Z-VAD-fmk (25 μm), or ferrostatin-1 and Z-VAD-fmk, and the number of viable cells was quantified. C, control and β4-depleted cells were detached for 24 h with 0.5% methylcellulose in the presence of either DMSO, ferrostatin-1 (2 μm), Z-VAD-fmk (25 μm), or ferrostatin-1 and Z-VAD-fmk, and the number of viable cells was quantified. NS, not significant. **, p < 0.01, ***, p < 0.005.