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. 2018 Jun 22;293(33):12741–12748. doi: 10.1074/jbc.RA118.003017

Figure 5.

Figure 5.

PVRL4-mediated cell clustering is necessary for α6β4-dependent Src activation. A, SUM-159 and Hs578t control cells were detached for 24 h in the presence of either IgG or a PVRL4 Ab, and the number of viable cells was quantified. B, SUM-159 and Hs578t β4-depleted cells were detached for 24 h in the presence of either IgG and DMSO, PVRL4 Ab, PVRL4 Ab and ferrostatin-1 (2 μm), or PVRL4 and Z-VAD-fmk (25 μm), and the number of viable cells was quantified. C, MCF10-A, SUM-159 and Hs578t control cells were detached for 2 h in the presence of either IgG or a PVRL4-blocking Ab, and phosphorylation of Src (Tyr-418) was assessed by immunoblotting. D, cells (MCF10-A and SUM-159) were assessed for viability after 24 h of detachment (clustered conditions) in the presence of either DMSO, PP2 (10 μm), or PP2 in combination with either ferrostatin-1 (2 μm) or Z-VAD-fmk (25 μm). **, p < 0.01, ***, p < 0.005.