Figure 3.
Pre-BCR is up-regulated in Hrd1 KO pre–B cells. A, VpreB (left) and λ5 (right) expression in WT and Hrd1 KO (KO) B220LOIgM− BM cells. B, percentage of VpreB and λ5 expression B220LOIgM− cells as analyzed in A. C, MFI of VpreB and λ5 as analyzed in A. D, μHC chain expression in B220LOIgM− pro–B and pre–B cells, B220LOIgM+ immature B cells, and B220HIIgM+ mature B cells. E, percentage of μHC+ cells as analyzed in D. F, MFI of μHC in μHC+ cells as measured in E. Error bars represent S.D. n = 7. *, p < 0.05; **, p < 0.01; ***, p < 0.001.