Figure 5.

AR binds to KIF5B via the N-terminal domain. A, a series of AR deletion constructs were generated by deleting progressively from the C and N termini. B and C, these constructs were expressed in COS-7 cells and analyzed by the in vitro pulldown assay with KIF5B-tail–coated magnet beads. Western blots were probed with the N-20 (B) and C-19 (C) antibodies, which recognized the N and C termini of AR, respectively. Control pulldowns were performed with a mouse IgG. H, Hinge domain; LBD, ligand-binding domain; N, NTD only; ND, NTD and DBD; NDH, NTD plus DBD and H; DHL, DBD plus H and LBD; HL, H and LBD.