Figure 4.

Fructose-1,6-bisphosphate-induced inhibition of respiration on permeabilized spheroplasts. A, fructose-1,6-bisphosphate-induced inhibition of respiration on permeabilized spheroplasts isolated from WT (black) and Δhxk2 (red) cells. B, fructose-1,6 bisphosphate-induced inhibition of respiration on permeabilized spheroplasts isolated from WT (black) and HAP4p-overexpressing (red) cells. Respiratory rates were measured as described under “Experimental procedures.” Results are mean ± S.E. of at least three separate experiments. The values at F1,6bp = 10 mm are statistically significantly different from the value at F1,6bP = 0 mm for each strain (WT, hxk2, and ΔHAP4OX).