Figure 6.

Study of the Crabtree effect in galactose-grown cells without (white) or with (black) glucose. A, glucose-induced decrease in respiratory rate. B, fructose-1,6-bisphosphate accumulation. C, glucose-6-phosphate modulation in cells. D, data from B and C were used to calculate the G6P/F1,6bP ratio. Respiratory rates, fructose-1,6-bisphosphate, and glucose-6-phosphate were measured as described under “Experimental procedures.” Results are mean ± S.E. of at least three separate experiments.