Table 1.

Inhibition, binding, and hydrolysis constants of APPI_WT and APPI-4M

APPI variant	Ki a	KDb	konb	koffb	Turnover timec
	nm	nm	m−1 s−1	s−1	h
APPIWT	2.24 ± 0.11d	5.29	(1.96 ± 0.01) × 105	(10.40 ± 0.02) × 10−4	16.62 ± 0.05
APPI-4M	0.16 ± 0.06	0.04	(3.17 ± 0.01) × 105	(1.15 ± 0.06) × 10−5	230.07 ± 40.90

^a Data were determined by slow tight binding inhibition assays.

^b Data were determined by SPR.

^c Data were determined by HPLC.

^d Data were previously reported in Ref. 50.