Table 1.

Thermodynamic parameters of proteases produced by representative isolates cultured at 28 and 7 °C

Cultured temp (°C)	Isolate	Temp		1/temp (×10−3 K−1)	k (min−1)	D (min)	t 1/2 (min)	E a (kJ/mol)	∆H # (kJ/mol)	∆S # (J/(mol·K))	∆G # (kJ/mol)
		(°C)	(K)
28	Pseudomonas fluorescens (d)	70	343	2.92	0.013	171.17	50.48	48.33	45.48	−124.86	88.24
		80	353	2.83	0.021	107.82	30.91		45.40	−117.95	87.08
		90	363	2.75	0.035	65.79	18.81		45.32	−111.38	85.82
	Pseudomonas azotoformans (a)	70	343	2.92	0.012	177.80	52.78	49.44	46.59	−122.23	88.45
		80	353	2.83	0.021	111.17	30.71		46.51	−114.81	87.08
		90	363	2.75	0.033	69.40	18.67		46.43	−108.74	85.97
	Serratia grimesii	70	343	2.92	0.010	225.75	66.66	53.72	50.87	−111.14	88.93
		80	353	2.83	0.017	135.19	39.30		50.79	−104.29	87.64
		90	363	2.75	0.030	77.59	22.03		50.71	−97.65	86.22
	Chryseobacterium polytrichastri	70	343	2.92	0.019	122.27	33.66	33.64	30.79	−164.89	87.26
		80	353	2.83	0.026	88.22	22.18		30.71	−157.94	86.52
		90	363	2.75	0.038	59.87	15.56		30.63	−151.20	85.61
	Flavobacterium frigidimaris (b)	70	343	2.92	0.017	133.05	37.16	36.72	33.35	−158.26	87.55
		80	353	2.83	0.026	90.28	23.61		33.27	−149.79	86.52
		90	363	2.75	0.036	63.42	16.44		33.19	−144.54	85.75
7	Pseudomonas fluorescens (d)	70	343	2.92	0.014	166.37	47.73	44.92	42.07	−134.26	88.05
		80	353	2.83	0.022	106.67	30.59		41.99	−127.24	86.95
		90	363	2.75	0.035	65.21	18.23		41.91	−120.75	85.82
	Pseudomonas azotoformans (a)	70	343	2.92	0.013	176.21	51.21	46.90	44.05	−129.03	88.24
		80	353	2.83	0.021	110.53	30.07		43.97	−122.00	87.08
		90	363	2.75	0.034	68.41	18.05		43.89	−115.53	85.90
	Serratia grimesii	70	343	2.92	0.010	220.51	58.46	53.72	50.87	−111.14	88.93
		80	353	2.83	0.017	133.59	37.70		50.79	−104.29	87.64
		90	363	2.75	0.030	77.13	21.58		50.71	−97.65	86.22
	Chryseobacterium polytrichastri	70	343	2.92	0.020	116.34	32.00	31.33	28.48	−171.23	87.12
		80	353	2.83	0.026	87.40	21.36		28.40	−164.48	86.52
		90	363	2.75	0.038	59.83	15.24		28.32	−157.55	85.61
	Flavobacterium frigidimaris (b)	70	343	2.92	0.018	130.05	35.46	34.23	31.38	−163.58	87.40
		80	353	2.83	0.026	90.02	22.71		31.30	−156.27	86.52
		90	363	2.75	0.036	63.53	16.24		31.22	−149.96	85.75