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. 2018 Aug;19(8):630–642. doi: 10.1631/jzus.B1700352

Table 2.

Thermodynamic parameters of lipases produced by representative isolates cultured at 28 and 7 °C

Cultured temp (°C) Isolate Temp
1/temp (×10−3 K−1) k (min−1) D (min) t 1/2 (min) E a (kJ/mol) ∆H # (kJ/mol) ∆S # (J/(mol·K)) ∆G # (kJ/mol)
(°C) (K)
28 Acinetobacter johnsonii (b) 70 343 2.92 0.033 70.17 21.56 27.24 24.39 −179.38 85.82
80 353 2.83 0.041 56.64 15.46 24.31 −172.69 85.33
90 363 2.75 0.058 39.41 10.60 24.23 −165.77 84.51
Acinetobacter pakistanensis 70 343 2.92 0.031 75.43 23.96 28.73 25.45 −176.75 85.98
80 353 2.83 0.040 58.23 16.32 25.37 −169.88 85.40
90 363 2.75 0.056 41.06 11.40 25.29 −163.10 84.60
Pseudomonas fluorescens (c) 70 343 2.92 0.051 45.55 13.44 21.51 18.66 −192.81 84.69
80 353 2.83 0.064 36.13 10.10 18.58 −185.65 84.18
90 363 2.75 0.079 29.07 8.11 18.50 −169.62 83.74
Pseudomonas azotoformans (a) 70 343 2.92 0.041 56.42 16.87 25.94 23.09 −181.51 85.25
80 353 2.83 0.055 41.59 11.67 23.01 −174.21 84.57
90 363 2.75 0.070 32.74 8.60 22.94 −167.97 84.02

7 Acinetobacter johnsonii (b) 70 343 2.92 0.033 69.92 21.31 27.24 24.39 −179.38 85.82
80 353 2.83 0.041 56.13 15.20 24.31 −172.69 85.33
90 363 2.75 0.058 39.55 10.62 24.23 −165.77 84.51
Acinetobacter pakistanensis 70 343 2.92 0.032 72.74 21.66 28.20 25.35 −176.81 85.90
80 353 2.83 0.040 57.44 15.77 25.27 −170.17 85.40
90 363 2.75 0.057 40.69 11.28 25.19 −163.27 84.56
Pseudomonas fluorescens (c) 70 343 2.92 0.052 44.31 12.78 21.03 18.18 −194.06 84.64
80 353 2.83 0.065 35.41 9.67 18.10 −186.89 84.14
90 363 2.75 0.079 28.98 7.77 18.02 −180.62 83.70
Pseudomonas azotoformans (a) 70 343 2.92 0.042 55.34 16.23 25.46 22.61 −182.73 85.19
80 353 2.83 0.056 41.19 11.53 22.53 −175.43 84.52
90 363 2.75 0.071 32.60 8.47 22.45 −169.21 83.98