Table I.
Sub-fractionation of organelles from Chlamydomonas reinhardtii
| Fraction | Chlorophyll | Protein | Rubisco | Cyt c Oxidase | Choline P Transferase | PEP Carboxylase | DGTS Synthesis, Total Activity | DGTS Synthesis, Specific Activity |
|---|---|---|---|---|---|---|---|---|
| % of total | ||||||||
| 760g ppt | 88.27 | 50.50 | 76.10 | 64.15 | 0.95 | 5.38 | 66.46 | 19.64 |
| 3,000g ppt | 6.21 | 5.26 | 2.54 | 11.96 | 9.80 | 0.95 | 5.42 | 12.30 |
| 10,000g ppt | 3.14 | 2.87 | 1.03 | 17.48 | 6.83 | 0.85 | 4.90 | 19.05 |
| 100,000g ppt | 2.38 | 4.72 | 2.43 | 4.16 | 82.25 | 3.13 | 23.22 | 49.00 |
| 100,000g aup | 0.00 | 36.35 | 17.89 | 2.25 | 0.16 | 89.70 | 0.00 | 0.00 |
Homogenization, organelle fractionation, and enzyme assays were as described in “Material and Methods.” Activities are presented as percentage of total activity in all fractions. Cyt c, Cytochrome c; PEP, phosphoenolpyruvate; ppt, precipitate; sup, supernatant.