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. 2018 Aug 20;8:12481. doi: 10.1038/s41598-018-31019-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Collected samples from (a) the inner wall (IW) outlet and (b) the outer wall (OW) outlet (scale bar is 200 µm). (c) monolayer culture in a 6-well plate in day 5 and (d) PrestoBlue assay displays semi-quantitative growth of harvested BM-hMSCs compared to control sample. (e) Viability of harvested BM-hMSCs from the OW outlet at pass (I,II) and the IW outlet at pass (II) compared to initial viability. (f) Yield gains from the OW outlet at first and second pass and loss from the IW outlet at second pass. (g) Multipotency assay of harvested BM-hMSCs using Oil Red O, Alizarin red and Alcian blue solution to stain lipid droplets (adipocytes, left panel), calcium deposits (osteoblasts, central panel), and extracellular matrix proteins (chondrocytes, right panel) respectively.