Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 20;8:12471. doi: 10.1038/s41598-018-30763-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

TDZ inhibits NF-κB nuclear translocation and suppressed the induction of NF-kB dependent pro-inflammatory signatures in RAW 264.7 macrophages. (A) Isolated cytoplasmic and nuclear fractions were probed for p65 by immunoblot before and after LPS (250 ng/ml) for 15 min, GAPDH and HDAC1 are loading controls for cytoplasmic or nuclear protein, respectively. (B–E) qPCR quantification of NF-kB dependent genes including iNOS, TNFα, IL1β, and IL6 treated with LPS or LPS + TDZ for the indicated time. The treatment of macrophages with TDZ significantly suppressed the transcription of NF-kB dependent genes. Values were normalized to GAPDH. P values were determined by Student’s T-test; ^***P < 0.005, <0.005^**P < 0.05, ^§§<0.001.