Table. 1.
De novo ceramide synthesis in H9c2 cells
| Crude membrane | Lysosome/mitochondria | |||
|---|---|---|---|---|
| NG | LG | NG | LG | |
| Control | 32.81 ± 1.9 | 14.27 ± 0.62 | 1.45 ± 1.27 | 1.14 ± 0.79 |
| DHA | 54.26 ± 1.73* | 20.85 ± 0.87 | 470.73 ± 15.94* | 0.25 ± 0.09 |
| 19,20-EDP | 50.50 ± 4.63* | 16.44 ± 0.52 | 601.73 ± 23.9* | 0.40 ± 0.21 |
| 19,20-EDP+myriocin | 18.23 ± 7.57† | 11.78 ± 0.24 | 64.20 ± 63.85† | 0.37 ± 0.22 |
LC/MS analysis was employed to measure ceramide levels in H9c2 cells cultured in either normal (25 mM) or low (5.5 mM) glucose-containing media
Ceramide content in crude membrane and lysosomal/mitochondria fractions were assessed. Cells were treated with or without DHA (100 μΜ), 19,20-EDP (1 μΜ) and myriocin (1 μΜ) for 24 h
Values are expressed as mean ± SEM (n = 4)
*P < 0.05 vs. control
†P < 0.05 vs. 19.20-EDP (NG)